Recombinant Protein Expression Services

We provide recombinant protein purification services for multiple expression systems, covering samples derived from prokaryotic (E. coli), yeast, insect cells, mammalian cells and cell-free expression systems. We tailor optimal lysis methods, purification strategies and buffer systems for each expression platform to guarantee the purity, bioactivity and stability of target proteins, fully meeting the requirements of downstream experiments.

Customers may submit samples in various forms, including bacterial pellets/cell pellets, culture supernatants, lysate supernatants and crude purification samples. We generally recommend clients to provide complete sample information (expression system, tag type, theoretical molecular weight, buffer composition and intended application). Based on these details, we will evaluate the optimal purification workflow and offer guidance on sample pretreatment to minimize the risk of purification failure.

Tags are not an absolute requirement. Tagged proteins (such as His, GST, Strep, FLAG, etc.) facilitate the establishment of efficient and stable affinity purification workflows. For untagged proteins, we can combine multiple purification strategies including ion exchange chromatography, hydrophobic interaction chromatography and size-exclusion chromatography. Customized purification protocols will be developed based on the target protein’s isoelectric point, molecular weight and stability.

Yes. For proteins intended for cell or animal studies, we offer dedicated endotoxin removal protocols as well as endotoxin quantification testing services. Endotoxin control is especially critical for prokaryotically expressed proteins. We take rigorous measures throughout the entire purification workflow to minimize endotoxin contamination and prevent secondary endotoxin exposure.