Polyclonal Ab Production

Polyclonal Ab Production

Ipodix Biotech: Polyclonal Antibody (Ab) Production Service

Polyclonal antibodies, capable of recognizing multiple epitopes with short production cycles and cost-effectiveness, are widely used in basic research, diagnostic development, and drug discovery. Ipodix Biotech delivers efficient and reliable polyclonal antibody production services, covering antigen design, animal immunization, antibody purification, and functional validation to help clients rapidly obtain highly specific antibodies.


Core Advantages

Ipodix optimizes antigen design and immunization strategies to ensure superior antibody performance:
● ‌Broad antigen compatibility‌: Supports proteins, peptides, haptens (e.g., hormones, drug molecules), and complex antigens (e.g., viral particles).
● ‌High affinity & titer‌: Utilizes multi-host immunization (rabbit, goat, guinea pig) with iterative titer monitoring to achieve titers ≥1:100,000.
● ‌Flexible formats‌: Provides crude serum, purified IgG (≥95% purity), or labeled antibodies (HRP, biotin, etc.).
● ‌Fast delivery‌: Standard timeline: 8–12 weeks; expedited services available (minimum 6 weeks).


Workflow & Deliverables

Service ModuleTimelineDeliverables
Antigen Design & Synthesis2–4 weeksAntigen design plan, synthesis QC report.
Animal Immunization & Titer Monitoring6–8 weeksImmunization protocol, serum titer data.
Serum Collection & Purification1–2 weeksCrude serum or purified IgG with purity report.
Functional Validation (Optional)2–3 weeksWB/ELISA/IHC application test data.

Host Selection & Applications

Ipodix recommends immunization hosts based on antigen properties:

HostAdvantagesApplications
RabbitStrong immune response, high yield, cost-effective.Protein/peptide antigens, diagnostic development.
GoatLarge serum volume, long-term supply.Industrial-scale production, multi-batch use.
Guinea PigSensitive to weak immunogens.Small molecule detection, cross-reactivity studies.
ChickenProduces IgY antibodies, low mammalian cross-reactivity.Experiments avoiding mammalian system interference.

Applications

FieldExamples
Basic ResearchWestern Blot, immunohistochemistry (IHC), flow cytometry.
Diagnostic DevelopmentInfectious disease detection, autoimmune diagnostic kits.
Drug DiscoveryTarget validation, neutralizing antibody screening.
Agriculture & EnvironmentPesticide residue detection, pathogen screening.
Industrial QCMonitoring impurities in biomanufacturing.

Quality Assurance

Ipodix ensures batch-to-batch consistency through stringent QC protocols:
● ‌Titer validation‌: ELISA with sensitivity ≥1:10,000.
● ‌Purity analysis‌: SDS-PAGE and HPLC (IgG purity ≥95%).
● ‌Specificity validation‌: Cross-reactivity tests, antigen adsorption.
● ‌Safety‌: Endotoxin <1 EU/mg, microbial testing compliant with international standards.

Performance Guarantee‌: Free re-immunization or full refund if specifications are unmet.


Collaboration Process

  1. Requirement Consultation‌: Provide target details, applications, and host preferences.

  2. Protocol Design‌: Receive antigen design proposal and quote within 48 hours.

  3. Agreement Finalization‌: Confirm deliverables, timeline, and acceptance criteria.

  4. Project Execution‌: Regular updates on immunization progress and data.

  5. Delivery & Support‌: Antibody samples, QC reports, and 12-month technical support.


Why Choose Ipodix?

● ‌Proven Expertise‌: 2000+ completed projects across oncology, infectious diseases, and rare targets.
● ‌Technical Mastery‌: Specialized in complex antigens (membrane proteins, glycoproteins).
● ‌Scalable Solutions‌: Milligram-scale research to gram-scale production.
● ‌Transparency‌: Real-time data access and collaborative decision-making.


Unlock Reliable Antibody Solutions Today!
Contact the Ipodix technical team via our website or hotline to discuss your project.