Analytical Data
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基因名
THBS1
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简介
The THBS1 protein is an adhesion glycoprotein that controls multiple cellular processes and mediates cell-cell and cell-matrix interactions. It plays multifunctional roles in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, senescence, self-renewal, stemness, and cardiovascular/metabolic homeostasis. THBS1 Protein, Human (HEK293, His) is the recombinant human-derived THBS1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF183 Protein is immobilized at 1 µg/mL (100 µL/well) can bind Human THBS1 Protein. The ED50 for this effect is 300-600 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF183 Protein is immobilized at 1 µg/mL (100 µL/well) can bind Human THBS1 Protein. The ED50 for this effect is 366.9 ng/mL.
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别名
Thrombospondin-1; THBS1; TSP; TSP1
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种属
Human
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表达系统
HEK293
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标签
C-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P07996
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表达区间
N19-P1170
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氨基酸序列
NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP
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蛋白长度
Full Length of Mature Protein
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分子量
130 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Thrombospondin-1 (THBS1) is a multifaceted glycoprotein that plays a crucial role in various biological processes, including cell adhesion, migration, and angiogenesis. It is primarily expressed by platelets and has been implicated in wound healing, immune response, and tumor progression. Given its significant involvement in these physiological and pathological processes, THBS1 has garnered considerable attention as a potential therapeutic target in cancer and vascular diseases. The reconstruction of THBS1 protein through recombinant DNA technology has provided researchers with valuable tools to study its structural and functional properties in greater detail. This approach allows for the generation of large quantities of pure, biologically active THBS1 for in vitro and in vivo studies. Additionally, recombinant THBS1 can be utilized in functional assays to decipher its interactions with other proteins and cells, thereby elucidating its role in various signaling pathways. Understanding the mechanisms by which THBS1 exerts its effects on cellular behaviors such as migration and proliferation may lead to novel interventions for diseases characterized by abnormal angiogenesis and cell growth. Furthermore, the potential for THBS1 to serve as a biomarker for certain diseases or as a therapeutic agent itself opens new avenues for research and clinical applications. As such, the study of recombinant THBS1 protein not only enhances our understanding of its biological functions but also paves the way for innovative treatment strategies.
