Analytical Data
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基因名
EGF
- Application
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生物活性
The ED50 is <0>1.0 × 107 units/mg. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 60.44 pg/mL, corresponding to a specific activity is 1.65×107 units/mg.
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别名
rMuEGF; Pro-epidermal growth factor; Urogastrone
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种属
Mouse
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P01132
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表达区间
N977-R1029
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蛋白长度
Full Length of EGF
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分子量
6-7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Epidermal Growth Factor (EGF) is a crucial protein that plays a significant role in cell growth, proliferation, and differentiation, particularly within the epithelial tissues. The discovery of EGF in the 1960s by Herbert Tobias and subsequently its characterization by Jeffrey Lane, which led to the awarding of the Nobel Prize to Stanley Cohen in 1986, underscored its importance in various biological processes. EGF is recognized for its ability to bind to the EGF receptor (EGFR), activating signaling pathways that are critical in normal cellular functions, yet it is also implicated in several pathological conditions, notably cancer. As research progressed, the recombinant production of EGF became a focal point for both therapeutic and cosmetic applications. Recombinant EGF is synthesized using biotechnology techniques, which enable the production of high-purity EGF for use in wound healing, tissue regeneration, and skin care products. The therapeutic potential of EGF has led to extensive studies examining its effects on skin rejuvenation, scar reduction, and treatment of various dermatological disorders. Furthermore, its role in enhancing tissue repair and regeneration has spurred research into its applications in regenerative medicine. By understanding the mechanisms of EGF action and its interactions at the molecular level, researchers aim to optimize its therapeutic efficacy and explore novel applications, such as in cancer treatment and tissue engineering, highlighting the protein's versatility and importance in both health and disease management.
