Analytical Data
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Gene name
TCN2/Transcobalamin-2
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简介
The TCN2/Transcobalamin-2 protein plays a key role as the primary vitamin B12 binding and transporter, ensuring efficient delivery of cobalamin to cells.Its complex interactions involve binding to CD320, specifically to the LDL receptor class A domain.TCN2/Transcobalamin-2 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived TCN2/Transcobalamin-2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED50 for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×10^4 Unit/mg. Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED50 for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×104 Unit/mg.
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Alternative Names
Transcobalamin-2; TC-2; TCII; Tcn2
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Species
Mouse
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O88968
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Expression Region
E19-W430
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AA Sequence
EFCVIPRIDSQLVEKLGQRLLPWMDRLSSEQLNPSVFVGLRLSSMQAGTKEDLYLHSLKIHYQQCLLRSTSSDDNSSCQPKLSGGSLALYLLALRANCEFFGSRKGDRLISQLKWFLEDEKKAIGHNHEGHPNTNYYQYGLSILALCVHQKRLHDSVVGKLLYAVEHDYFTYQGHVSVDTEAMAGLALTCLERFNFNSDLRPRITMAIETVREKILKSQAPEGYFGNIYSTPLALQMLMTSPASGVGLGTACIKAGTSLLLSLQDGAFQNPLMISQLLPILNHKTYLDLIFPDCQASRVMLVPAVEDPVHISEVISVTLKVASALSPYEQTFFVFAGSSLEDVLKLAQDGGGFTYGTQASLSGPYLTSVLGKDAGDREYWQLLRAPDTPLLQGIADYKPQDGETIELRLVRW
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Protein Length
Full Length of Mature Protein
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Molecular Weight
42.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TCN2, or Transcobalamin-2, is a key protein responsible for the cellular uptake and transport of vitamin B12 (cobalamin). It plays a vital role in maintaining cobalamin homeostasis and facilitating its biosynthetic processes. Mutations and polymorphisms in the TCN2 gene have been associated with various clinical conditions, including neurological disorders, cardiovascular diseases, and certain types of anemia, highlighting its significance in human health. Given the crucial role of TCN2 in vitamin B12 metabolism, research into its recombinant protein form has gained traction. Recombinant TCN2 can be utilized in studying the mechanisms of cobalamin transport and signaling in different cellular contexts. Additionally, it serves as a potential candidate for developing therapeutic strategies aimed at addressing vitamin B12 deficiency and associated disorders. By producing and analyzing recombinant TCN2, researchers aim to elucidate its structure-function relationships, investigate its interactions with cellular receptors, and explore its role in cobalamin-mediated pathways. The findings from such studies are essential not only for understanding basic biological processes but also for paving the way for new approaches in clinical nutrition and therapeutic intervention.
