Analytical Data
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基因名
GPR81
- Application
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别名
HCAR1; HCA1; FKSG80; GPR104; TA-GPCR; LACR1; Lactate Receptor 1; Hydroxycarboxylic acid receptor 1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BXC0
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表达区间
Ser250~His346
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分子量
15kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPR81, also known as the hydroxycarboxylic acid receptor 1 (HCA1), is a G protein-coupled receptor (GPCR) predominantly expressed in adipose tissue and other metabolic tissues. It is recognized for its role in the regulation of energy metabolism and has garnered attention due to its involvement in various physiological processes, including lipolysis and insulin sensitivity. Research has shown that GPR81 is activated by lactate, a byproduct of anaerobic metabolism, indicating its potential role in metabolic adaptation to exercise and hypoxia. The elucidation of GPR81 signaling pathways has substantial implications for understanding metabolic disorders, including obesity and type 2 diabetes. Given its therapeutic potential, the production of recombinant GPR81 protein is crucial for studying its structure-function relationships, pharmacological properties, and interaction with various ligands. By generating and characterizing recombinant GPR81, researchers aim to advance the understanding of its biological functions and explore its potential as a drug target for metabolic diseases. The recombinant protein can be utilized in binding assays, functional studies, and structure determination, providing invaluable insights that may aid in the development of novel therapeutic strategies targeting metabolic dysfunctions.
