Analytical Data
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基因名
CPOX
- Application
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别名
CPOX；CPO；CPX;Oxygen-dependent coproporphyrinogen-III oxidase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P36551
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表达区间
111-454aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSTSLGRPEEEEDELAHRCSSFMAPPVTD LGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGG GGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLP FCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGNKQWWFGGGCDLTPTY LNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGI FFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQL RRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENS KEAEILEVLRHPRDWVR
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分子量
42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CPOX (Coproporphyrinogen oxidase) is a critical enzyme involved in the heme biosynthesis pathway, catalyzing the conversion of coproporphyrinogen III to protoporphyrinogen IX. Mutations in the CPOX gene can lead to hereditary coproporphyria, a condition characterized by the accumulation of porphyrins, which can result in severe neurological and gastrointestinal disturbances. Given the importance of heme in cellular metabolism and the potential impact of CPOX mutations on human health, extensive research has been dedicated to understanding the structure and function of the CPOX protein. Recombination technologies have enabled the production of CPOX as a recombinant protein, providing a valuable tool for biochemical studies. Investigating the protein’s enzymatic activity, regulatory mechanisms, and interaction with substrates and inhibitors are essential to elucidate its role in heme synthesis and to develop potential therapeutic strategies for porphyrias. Furthermore, understanding the structural dynamics of CPOX contributes to the broader field of protein engineering and offers insights into the design of novel biomolecules with applications in biomedicine and biotechnology. As recombinant CPOX becomes increasingly accessible, it is expected to serve as a basis for advancements in the diagnosis and treatment of related disorders, ultimately enhancing our understanding of porphyrin metabolism and its implications for human health.
