Analytical Data
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Gene name
SLC1A5
- Application
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Alternative Names
SLC1A5；Amino acid transporter
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15758
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Expression Region
1-541aa
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AA Sequence
MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM
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Molecular Weight
56.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC1A5, also known as System L amino acid transporter 1 (LAT1), is a crucial membrane protein involved in the transport of neutral amino acids, particularly leucine and other large, hydrophobic amino acids, across the plasma membrane of cells. This transporter plays a significant role in cellular metabolism, protein synthesis, and overall cellular homeostasis, making it a focal point of research in various fields including cancer biology, neurobiology, and metabolic disorders. Aberrant expression of SLC1A5 has been linked to several human diseases, especially tumors where it supports the increased demand for nutrients in rapidly proliferating cancer cells. In addition, the modulation of SLC1A5 has been identified as a potential therapeutic target, as its inhibition could restrict amino acid supply to malignant cells, thereby impeding their growth and survival. Understanding the structure and function of SLC1A5, including its substrate specificity and transport mechanisms, is critical for the development of effective inhibitors or modulators. Recent advances in recombinant protein technology have facilitated the purification and characterization of SLC1A5, paving the way for detailed studies on its function and its role in disease. This research contributes to a greater understanding of amino acid transport processes and their implications in health and disease, ultimately leading to novel therapeutic strategies targeting SLC1A5 in oncological and other metabolic diseases.
