Analytical Data
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基因名
HDX
- Application
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别名
CXorf43 ; D030011N01Rik; FLJ30678; Hdx; HDX_HUMAN; Highly divergent homeobox; MGC126769; MGC126771; OTTHUMP00000062139
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7Z353
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表达区间
1-690aa
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氨基酸序列
MNLRSVFTVEQQRILQRYYENGMTNQSKNCFQLILQCAQETKLDFSVVRTWVGNKRRKMSSKNSESGTATTGTSLSAPDITVRNVVNIARPSSQQSSWTSANNDVIVTGIYSPASSSSRQGTNKHTDTQITEAHKIPIQKTATKNDTEFQLHIPVQRQVAHCKNASLLLGEKTIILSRQTSVLNAGNSVFNHAKKNYGNSSVQASEMTVPQKPSVCHRPCKIEPVGIQRSYKPEHTGPALHNLCGQKPTIRDPYCRTQNLEIREVFSLAVSDYPQRILGGNAPQKPSSAEGNCLSIAMETGDAEDEYAREEELASMRAQIPSYSRFYESGSSLRAENQSTTLPGPGRNMPNSQMVNIRDMSDNVLYQNRNYHLTPRTSLHTASSTMYSNTNPLRSNFSPHFASSNQLRLSQNQNNYQISGNLTVPWITGCSRKRALQDRTQFSDRDLATLKKYWDNGMTSLGSVCREKIEAVATELNVDCEIVRTWIGNRRRKYRLMGIEVPPPRGGPADFSEQPESGSLSALTPGEEAGPEVGEDNDRNDEVSICLSEGSSQEEPNEVVPNDARAHKEEDHHAVTTDNVKIEIIDDEESDMISNSEVEQVNSFLDYKNEEVKFIENELEIQKQKYFKLQTFVRSLILAMKADDKEQQQALLSDLPPELEEMDFNHASLEPDDTSFSVSSLSEKNVSESL
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分子量
103.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of HDX (hydrogen-deuterium exchange) recombinant proteins is critical in understanding protein structure, dynamics, and interactions at the molecular level. Protein folding, stability, and functionality are essential aspects of biochemistry, and HDX serves as a powerful technique to probe these characteristics. In HDX experiments, hydrogen atoms in specific regions of the protein are replaced with deuterium, allowing researchers to track the exchange rates of amide protons in peptides. This technique provides insights into protein dynamics, accessibility, and conformational changes in response to various environmental factors. Furthermore, HDX is particularly valuable for studying protein-ligand interactions, as it can reveal binding-induced structural rearrangements and conformational states. With advancements in mass spectrometry, HDX analysis has become more refined, enabling high-resolution mapping of protein regions that participate in binding and interactions. Therefore, research on HDX recombinant proteins offers significant implications for drug design, therapeutic development, and the understanding of various diseases related to protein misfolding or dysfunction. As such, the ongoing exploration of HDX methodologies and their application to recombinant proteins is paramount in the fields of structural biology and biochemistry.
