Analytical Data
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基因名
THRa
- Application
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别名
THRa；AIB1；BHLHE42；RAC3；Nuclear receptor coactivator 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10827
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表达区间
1-490aa
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氨基酸序列
MEQKPSKVECGSDPEENSARSPDGKRKRKNGQCSLKTSMSGYIPSYLDKDEQCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPTYSCKYDSCCVIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEQNRERRRKEEMIRSLQQRPEPTPEEWDLIHIATEAHRSTNAQGSHWKQRRKFLPDDIGQSPIVSMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEMAVKREQLKNGGLGVVSDAIFELGKSLSAFNLDDTEVALLQAVLLMSTDRSGLLCVDKIEKSQEAYLLAFEHYVNHRKHNIPHFWPKLLMKEREVQSSILYKGAAAEGRPGGSLGVHPEGQQLLGMHVVQGPQVRQLEQQLGEAGSLQGPVLQHQSPKSPQQRLLELLHRSGILHARAVCGEDDSSEADSPSSSEEEPEVCEDLAGNAASP
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分子量
68.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of THRa (thyroid hormone receptor alpha) recombinant proteins has gained significant attention due to the pivotal role thyroid hormones play in regulating various physiological processes, including metabolism, development, and growth. THRa is a member of the nuclear receptor superfamily, and it functions primarily as a ligand-dependent transcription factor, modulating the expression of target genes upon binding with thyroid hormones like thyroxine (T4) and triiodothyronine (T3). Research has shown that dysregulation of THRa can lead to various disorders, including congenital hypothyroidism and resistance to thyroid hormone syndrome. The development of recombinant THRa proteins allows for detailed studies of its structural and functional properties, facilitating insights into ligand binding, receptor conformational changes, and the molecular mechanisms underlying its action in target tissues. Furthermore, recombinant THRa proteins are valuable tools in drug discovery, enabling the screening of potential therapeutic compounds that could modulate receptor activity. By employing techniques such as site-directed mutagenesis and protein expression in host systems, researchers can generate modified versions of THRa to elucidate the receptor’s biological functions and its interactions with co-regulators. Overall, the advancements in THRa recombinant protein research hold promise for understanding thyroid hormone signaling pathways and developing novel therapeutic strategies for related disorders.
