Analytical Data
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基因名
GSDMD
- Application
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别名
GSDMD；DFNA5L；GSDMDC1；Gasdermin-D
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P57764
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表达区间
1-484aa
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氨基酸序列
MGSAFERVVRRVVQELDHGGEFIPVTSLQSSTGFQPYCLVVRKPSSSWFW KPRYKCVNLSIKDILEPDAAEPDVQRGRSFHFYDAMDGQIQGSVELAAPG QAKIAGGAAVSDSSSTSMNVYSLSVDPNTWQTLLHERHLRQPEHKVLQQL RSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHL SQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRS TSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKE LELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAG AVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLG PLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELG EDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
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分子量
79 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Gasdermin D (GSDMD) is a member of the gasdermin family of proteins and plays a crucial role in the process of pyroptosis, a form of programmed cell death associated with inflammation. Research has shown that GSDMD is activated by inflammatory caspases, particularly caspase-1 and caspase-11, which cleave the protein to produce its active N-terminal fragment. This fragment then forms pores in the cell membrane, leading to cell lysis and the release of pro-inflammatory cytokines, such as IL-1β and IL-18. Understanding the structure and function of GSDMD is essential for elucidating its role in various pathophysiological conditions, including infections and inflammatory diseases. The generation of recombinant GSDMD proteins has facilitated detailed studies on its biochemical properties, including pore formation and interactions with other cellular components. Recent studies suggest that GSDMD may also be implicated in non-canonical inflammatory pathways and certain cancer processes, further emphasizing its importance in immune responses and disease mechanisms. The development of GSDMD-targeted therapeutics is an evolving area of interest, with the potential to mitigate excessive inflammation and tissue damage associated with various diseases. Thus, research on recombinant GSDMD proteins is vital for advancing our understanding of its biological functions and therapeutic applications in regulating inflammation and cell death.
