Analytical Data
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基因名
NEK10
- Application
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别名
NEK10; Serine/threonine-protein kinase Nek10; EC 2.7.11.1; Never in mitosis A-related kinase 10; NimA-related protein kinase 10
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6ZWH5
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表达区间
1-474 aa
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氨基酸序列
MVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR
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分子量
79.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The NEK10 protein, a member of the NIMA-related kinase family, has garnered attention in recent years due to its potential implications in cell cycle regulation and various diseases, including cancer. NEK10 is involved in several critical cellular processes, such as mitosis and the DNA damage response, which positions it as a significant player in maintaining genomic stability. Research indicates that altered expression of NEK10 may contribute to tumorigenesis, highlighting its role as a potential biomarker or therapeutic target in cancer treatment. Additionally, studies have revealed that NEK10 may interact with other key proteins involved in cell signaling pathways, further implicating its importance in cellular physiology. Due to these associations, scientists are increasingly focused on studying NEK10's structure, function, and regulation. The recombinant production of NEK10 can enhance the understanding of its biochemical properties and interactions, facilitating the exploration of its role in disease and opening avenues for the development of novel therapeutic strategies. As research continues, the characterization of NEK10 through recombinant protein studies is expected to shed light on its mechanistic functions and contribution to human health, thereby advancing the field of molecular biology and cancer research.
