Analytical Data
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基因名
TARS2
- Application
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别名
TARS2；TARSL1；Threonine--tRNA ligase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BW92
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表达区间
369-718aa
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氨基酸序列
EHYQEDMFAVQPPGSDRPPSSQSDDSTRHITDTLALKPMNCPAHCLMFAHRPRSWRELPLRLADFGALHRAEASGGLGGLTRLRCFQQDDAHIFCTTDQLEAEIQSCLDFLRSVYAVLGFSFRLALSTRPSGFLGDPCLWDQAEQVLKQALKEFGEPWDLNSGDGAFYGPKIDVHLHDALGRPHQCGTIQLDFQLPLRFDLQYKGQAGALERPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPFQVVVIPVGSEQEEYAKEAQQSLRAAGLVSDLDADSGLTLSRRIRRAQLAHYNFQFVVGQKEQSKRTVNIRTRDNRRLGEWDLPEAVQRLVELQNTRVPNAEEIF
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分子量
55.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TARS2, or mitochondrial threonyl-tRNA synthetase 2, is an essential enzyme that plays a pivotal role in mitochondrial protein translation by facilitating the charging of tRNA with the amino acid threonine. Mutations in the TARS2 gene have been linked to various mitochondrial disorders, which often manifest in multi-systemic symptoms, including neurological deficits and myopathy. The study of TARS2 recombinant protein is crucial for understanding its structural and functional properties, particularly concerning how specific mutations affect enzyme activity and mitochondrial function. These insights are vital for developing potential therapeutic strategies aimed at mitigating the effects of TARS2-related mitochondrial dysfunctions. Research on TARS2 recombinant protein typically involves expressing and purifying the protein in model systems, followed by biochemical analyses to assess its activity, stability, and interactions with other mitochondrial components. By elucidating the mechanistic details of TARS2 function, scientists aim to uncover the underlying causes of the associated disorders, ultimately paving the way for genetic and therapeutic interventions that could improve patient outcomes. This research not only contributes to the broader field of mitochondrial biology but also enhances our understanding of the interplay between tRNA synthetases and mitochondrial health.
