Analytical Data
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基因名
ADAT1
- Application
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别名
ADAT 1; ADAT1; ADAT1_HUMAN; Adenosine deaminase acting on tRNA
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BUB4
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表达区间
1-502aa
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氨基酸序列
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ FK
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分子量
55.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
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Protein Description
ADAT1, or tRNA adenosine deaminase 1, is a crucial enzyme involved in the modification of tRNA, responsible for converting adenosine at position 37 to inosine, which plays a significant role in the accuracy and efficiency of protein translation. The study of ADAT1 has gained attention due to its implications in various biological processes and diseases. Abnormalities in tRNA modifications, including those mediated by ADAT1, have been linked to several disorders, including neurological conditions and certain cancers. The recombinant production of ADAT1 offers a valuable tool for investigating its enzymatic properties, structural characteristics, and functional roles in cellular metabolism. By utilizing recombinant DNA technology, researchers can produce large quantities of ADAT1, enabling detailed biophysical and biochemical studies. Understanding ADAT1's mechanisms and potential as a therapeutic target may provide insights into novel strategies for treating diseases associated with tRNA modification defects. Ultimately, the ongoing research on ADAT1 and its recombinant protein could pave the way for advancements in molecular medicine and the development of new biomolecular tools.
