Analytical Data
-
基因名
ANAPC4
- Application
-
别名
ANAPC 4; anapc4; Anaphase Promoting Complex Subunit 4
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9UJX5
-
表达区间
1-808aa
-
氨基酸序列
MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS
-
分子量
92.1 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human OR5D16 Protein，His
Cat：PA2000-9984
Recombinant Human ANAPC2 Protein，GST
Cat：PA2000-5473
Protein Description
ANAPC4, part of the Anaphase Promoting Complex/Cyclosome (APC/C), plays a crucial role in regulating the cell cycle, particularly during the transition from metaphase to anaphase. This multicatalytic E3 ubiquitin ligase complex is responsible for tagging specific proteins with ubiquitin, marking them for degradation by the proteasome. Such regulation is vital for ensuring proper chromosome segregation and maintaining genomic stability. Aberrations in the function of APC/C components, including ANAPC4, have been implicated in various cancers, underscoring their significance in tumorigenesis. Research on ANAPC4 has focused on its structural properties, interaction with other APC/C subunits, and the mechanisms underlying its role in targeted protein degradation. Understanding ANAPC4's function can provide insights into cell cycle regulation and potential therapeutic targets for cancer treatment. Recent studies employing recombinant protein technology have enabled a more detailed investigation of ANAPC4, facilitating the exploration of its biochemical activities and interactions with substrates, which is essential for developing strategies to manipulate its function in cancer cells. This research not only emphasizes the fundamental importance of ANAPC4 in cell division but also highlights its potential as a biomarker and therapeutic target in oncology.
