Analytical Data
-
基因名
PPM1J
- Application
-
别名
PPM1J; PPP2CZ; Protein phosphatase 1J; EC 3.1.3.16; Protein phosphatase 2C isoform zeta; PP2C-zeta
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q5JR12
-
表达区间
1-505 aa
-
氨基酸序列
MLNRVRSAVA HLVSSGGAPP PRPKSPDLPN AASAPPAAAP EAPRSPPAKA GSGSATPAKA VEARASFSRP TFLQLSPGGL RRADDHAGRA VQSPPDTGRR LPWSTGYAEV INAGKSRHNE DQACCEVVYV EGRRSVTGVP REPSRGQGLC FYYWGLFDGH AGGGAAEMAS RLLHRHIREQ LKDLVEILQD PSPPPLCLPT TPGTPDSSDP SHLLGPQSCW SSQKEVSHES LVVGAVENAF QLMDEQMARE RRGHQVEGGC CALVVIYLLG KVYVANAGDS RAIIVRNGEI IPMSREFTPE TERQRLQLLG FLKPELLGSE FTHLEFPRRV LPKELGQRML YRDQNMTGWA YKKIELEDLR FPLVCGEGKK ARVMATIGVT RGLGDHSLKV CSSTLPIKPF LSCFPEVRVY DLTQYEHCPD DVLVLGTDGL WDVTTDCEVA ATVDRVLSAY EPNDHSRYTA LAQALVLGAR GTPRDRGWRL PNNKLGSGDD ISVFVIPLGG PGSYS
-
分子量
54.8 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PPM1J, also known as protein phosphatase 2C family member J, is a serine/threonine protein phosphatase that plays a crucial role in various cellular processes by regulating phosphorylation states of target proteins. Its involvement in key pathways, including cellular stress response, cell cycle regulation, and apoptosis, has made it a subject of interest in cancer research and neurodegenerative diseases. Aberrant expression or mutations in PPM1J can disrupt normal cellular functions and contribute to disease progression. Recent studies have indicated that PPM1J may also participate in immune regulation and inflammatory responses, linking it to various autoimmune disorders. Given its significance, the exploration of PPM1J as a potential therapeutic target has gained momentum. This includes the generation of recombinant PPM1J proteins for functional assays and drug discovery efforts. Understanding the structural and functional properties of PPM1J through recombinant protein techniques provides insights into its mechanisms of action, potential interactions with other signaling pathways, and implications for disease treatment. Thus, research into PPM1J not only expands our understanding of protein phosphatases but also offers promising avenues for developing targeted therapies for diseases associated with dysregulated phosphorylation signaling.
