Analytical Data
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基因名
NR1I2
- Application
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别名
NR1I2；PXR；Nuclear receptor subfamily 1 group I member 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75469
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表达区间
1-434aa
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氨基酸序列
MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS
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分子量
69.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NR1I2, also known as Hepatic Nuclear Factor 4 alpha (HNF4α), is a key transcription factor that plays a crucial role in liver development, metabolism, and the regulation of various metabolic pathways. Research on NR1I2 has gained significant attention due to its involvement in drug metabolism and hepatotoxicity, as it regulates the expression of numerous genes responsible for the detoxification of xenobiotics and the metabolism of endogenous compounds. Understanding the function and regulatory mechanisms of NR1I2 is essential not only for elucidating the pathways involved in liver function but also for developing therapeutic strategies for liver-related diseases, including non-alcoholic fatty liver disease and drug-induced liver injury. Furthermore, NR1I2 has been implicated in the modulation of cholesterol and fatty acid metabolism, thereby influencing overall lipid homeostasis. The reconstitution of NR1I2 as a recombinant protein allows for detailed studies of its structural and functional properties, facilitating the investigation of ligand binding, DNA interaction, and the effects of post-translational modifications. This research holds promise for advancing our knowledge of liver biology and improving drug development processes by identifying potential targets for intervention in metabolic disorders. Consequently, the study of NR1I2 and its recombinant form represents a vital area of inquiry with significant implications for pharmacology and toxicology, as well as for understanding complex diseases linked to metabolic dysregulation.
