Analytical Data
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Gene name
hldE
- Application
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Alternative Names
hldE；rfaE；waaE；Bifunctional Protein HldE
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P76658
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Expression Region
1-477aa
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AA Sequence
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
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Molecular Weight
67.1kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HldE, a protein encoded by the hldE gene, is shown to be involved in the biosynthesis of a class of compounds critical for bacterial physiology and pathogenicity. This protein has gained attention due to its role in the metabolic pathways of certain bacteria, particularly in the production of unique secondary metabolites. Research has demonstrated that HldE participates in the synthesis of specific pigments and virulence factors, which are essential for bacterial survival and interaction with host organisms. Understanding the structure and function of HldE is vital, as it can provide insights into the mechanisms of bacterial pathogenesis and the development of potential therapeutic strategies. Additionally, studies involving HldE may contribute to the field of synthetic biology, where harnessing bacteria's metabolic capabilities could lead to innovative applications in biotechnology, including the production of biofuels, pharmaceuticals, and agricultural products. Consequently, characterizing the recombinant HldE protein would not only elucidate its biochemical roles but also enhance our understanding of bacterial metabolism and its applications in various industries. Through protein expression, purification, and functional assays, researchers aim to decipher the precise mechanisms by which HldE influences metabolic pathways and its overall impact on bacterial behavior, making it a significant target for both fundamental and applied research.
