Analytical Data
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基因名
NPC1
- Application
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别名
NPC1；NPC intracellular cholesterol transporter 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15118
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表达区间
151-250aa
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氨基酸序列
GFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQ APFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPK
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分子量
37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Niemann-Pick C1-like 1 protein (NPC1) is a crucial membrane protein primarily known for its role in cholesterol and lipid transport within the endosomal-lysosomal pathway. Mutations in the NPC1 gene are linked to Niemann-Pick disease type C, a severe neurodegenerative disorder characterized by cholesterol accumulation in various tissues, particularly the brain. Research into NPC1 has gained momentum due to its significant implications in cellular lipid homeostasis, disease progression, and potential therapeutic avenues. The recombinant expression of NPC1 protein allows for detailed structural and functional studies, which are essential for understanding its mechanisms and interactions at a molecular level. Additionally, NPC1 has garnered attention in the context of viral infections, including its involvement in the entry of several pathogens, making it a potential target for antiviral strategies. By generating and characterizing NPC1 recombinant proteins, researchers aim to elucidate its role in lipid metabolism, develop effective treatment options for Niemann-Pick disease, and investigate its relevance in infectious disease contexts, thereby contributing to a broader understanding of lipid biology and disease mechanisms.
