Analytical Data
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Gene name
RFFL
- Application
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Alternative Names
CARP-2; CARP2; Caspase regulator CARP2; Caspases 8 and 10 associated RING finger protein 2; caspases-8 and -10- associated RING finger protein 2; Caspases-8 and -10-associated RING finger protein 2; E3 ubiquitin protein ligase rififylin; E3 ubiquitin-protein ligase rififylin; Fring; FYVE RING finger protein Sakura; FYVE-RING finger protein Sakura; Rffl; RFFL_HUMAN; Rififylin; RING finger and FYVE like domain containing protein; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34 like; RING finger protein 34-like; RNF189; RNF34L
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WZ73
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Expression Region
1-363 aa
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AA Sequence
MWATCCNWFC LDGQPEEVPP PQGARMQAYS NPGYSSFPSP TGLEPSCKSC GAHFANTARK QTCLDCKKNF CMTCSSQVGN GPRLCLLCQR FRATAFQREE LMKMKVKDLR DYLSLHDIST EMCREKEELV LLVLGQQPVI SQEDRTRAST LSPDFPEQQA FLTQPHSSMV PPTSPNLPSS SAQATSVPPA QVQENQQANG HVSQDQEEPV YLESVARVPA EDETQSIDSE DSFVPGRRAS LSDLTDLEDI EGLTVRQLKE ILARNFVNYK GCCEKWELME RVTRLYKDQK GLQHLVSGAE DQNGGAVPSG LEENLCKICM DSPIDCVLLE CGHMVTCTKC GKRMNECPIC RQYVIRAVHV FRS
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Molecular Weight
40.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RFFL (Ring Finger Protein Like) is a crucial component in various cellular processes, including protein degradation, cell signaling, and apoptosis, due to its role as an E3 ubiquitin ligase. The study of RFFL is significant because it contributes to the understanding of ubiquitin-proteasome pathways, which are vital for maintaining cellular homeostasis and regulating protein levels within the cell. Dysregulation of these pathways is linked to several diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Recent research has shed light on the structural and functional characteristics of RFFL, revealing its potential as a therapeutic target. Through the manipulation of RFFL activity, researchers aim to exploit its mechanisms for novel treatment strategies. Additionally, RFFL's involvement in the regulation of key cellular proteins underscores its importance in various physiological and pathological contexts. Overall, the ongoing exploration of RFFL's functions and interactions holds promise for advancing our understanding of complex biological systems and the development of innovative therapeutic interventions.
