Analytical Data
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基因名
nrdA
- Application
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别名
nrdA；RR1；Ribonucleoside-diphosphate reductase large subunit
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00452
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表达区间
1-761aa
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氨基酸序列
MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPALYDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAEDAQDDLVPSIQDDGCESGACKI
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分子量
101.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NrdA is a crucial component of the ribonucleotide reductase (RNR) enzyme complex, which plays a pivotal role in DNA synthesis by catalyzing the reduction of ribonucleotides to deoxyribonucleotides. This process is essential for all living organisms as it provides the necessary building blocks for DNA replication and repair. The study of recombinant NrdA protein has garnered significant attention in biochemical and molecular biology research due to its implications in cancer biology and antibiotic resistance. As RNR is a target for cancer therapeutics and antibiotic development, understanding the structure and function of NrdA is vital for designing effective inhibitors. Additionally, the ability to produce NrdA as a recombinant protein allows for detailed biochemical assays, structural studies, and the exploration of its interaction with other proteins and substrates. Advances in recombinant DNA technology have facilitated the expression and purification of NrdA from various host systems, providing insights into its enzymatic mechanism and regulation. This research not only enhances our knowledge of fundamental cellular processes but also opens avenues for novel drug discoveries aimed at disrupting the RNR pathway in pathogenic organisms and cancer cells. The investigation of NrdA in various contexts, including its evolutionary conservation and potential post-translational modifications, continues to be a dynamic field, promising to unravel new dimensions in our understanding of nucleotide metabolism and its broader biological significance.
