Analytical Data
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Gene name
RHOT2
- Application
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Alternative Names
ARHT2 ; C16orf39 ; hMiro 2; hMiro-2; hMiro2; MIRO-2; MIRO2_HUMAN; Mitochondrial Rho GTPase 2; Ras homolog gene family member T2; RASL ; RHOT2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IXI1
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Expression Region
1-618 aa
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AA Sequence
MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ
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Molecular Weight
94.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RHOT2, a member of the Ras homolog family, is a GTPase that plays a vital role in various cellular processes, including mitochondrial dynamics, cell migration, and proliferation. Research on RHOT2 has gained momentum due to its involvement in critical biological functions and its potential implications in disease mechanisms, particularly in cancer and neurodegenerative disorders. Unlike other members of the Rho GTPase family, RHOT2 has unique regulatory mechanisms and subcellular localization, primarily associated with mitochondria. Understanding the signaling pathways and molecular interactions of RHOT2 provides insights into its function in cellular homeostasis and stress responses. Studies have demonstrated that RHOT2 can influence mitochondrial morphology and distribution, linking it to bioenergetics and apoptosis. Moreover, its overexpression has been correlated with tumor progression, making it a potential therapeutic target. The characterization of RHOT2 as a recombinant protein facilitates the exploration of its biochemical properties, interactions with downstream effectors, and role in cellular processes. This research contributes to the broader understanding of GTPases and their potential as regulatory nodes in cancer and neurodegenerative disease pathways, opening avenues for novel intervention strategies.
