Analytical Data
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基因名
cry3Aa
- Application
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别名
cry3Aa；bt13；cry3A；cryC；Pesticidal crystal Protein Cry3Aa
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A379
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表达区间
58-644aa
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氨基酸序列
DSSTTKDVIQKGISVVGDLLGVVGFPFGGALVSFYTNFLNTIWPSEDPWKAFMEQVEALMDQKIADYAKNKALAELQGLQNNVEDYVSALSSWQKNPVSSRNPHSQGRIRELFSQAESHFRNSMPSFAISGYEVLFLTTYAQAANTHLFLLKDAQIYGEEWGYEKEDIAEFYKRQLKLTQEYTDHCVKWYNVGLDKLRGSSYESWVNFNRYRREMTLTVLDLIALFPLYDVRLYPKEVKTELTRDVLTDPIVGVNNLRGYGTTFSNIENYIRKPHLFDYLHRIQFHTRFQPGYYGNDSFNYWSGNYVSTRPSIGSNDIITSPFYGNKSSEPVQNLEFNGEKVYRAVANTNLAVWPSAVYSGVTKVEFSQYNDQTDEASTQTYDSKRNVGAVSWDSIDQLPPETTDEPLEKGYSHQLNYVMCFLMQGSRGTIPVLTWTHKSVDFFNMIDSKKITQLPLVKAYKLQSGASVVAGPRFTGGDIIQCTENGSAATIYVTPDVSYSQKYRARIHYASTSQITFTLSLDGAPFNQYYFDKTINKGDTLTYNSFNLASFSTPFELSGNNLQIGVTGLSAGDKVYIDKIEFIPVN
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分子量
84.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cry3Aa is a member of the Cry protein family produced by the bacterium Bacillus thuringiensis (Bt), which has garnered significant attention due to its insecticidal properties against certain pests, particularly within the Coleoptera order, such as the Colorado potato beetle. The increasing demand for sustainable agricultural practices has highlighted the need for effective biopesticides that can mitigate the reliance on chemical pesticides, which often lead to environmental and health concerns. Research into the structural and functional aspects of Cry3Aa has revealed insights into its mechanism of action and potential for enhancing pest resistance in crops. The recombinant expression of Cry3Aa in various systems allows for detailed studies on its efficacy, stability, and safety. Additionally, efforts are underway to improve its potency and broaden its spectrum of activity through genetic engineering techniques, enabling the development of transgenic plants that incorporate Cry3Aa to achieve improved pest resistance. This research not only provides a sustainable solution for crop protection but also contributes to the understanding of insect-host interactions, laying the groundwork for future advancements in agricultural biotechnology. The exploration of Cry3Aa and its derivatives continues to be pivotal in addressing global food security challenges while promoting environmentally friendly farming practices.
