Analytical Data
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基因名
recF
- Application
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别名
recF；DNA replication and repair Protein RecF
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7H0
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表达区间
2-357aa
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氨基酸序列
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
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分子量
56.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RecF is a crucial protein involved in the DNA recombination and repair processes, particularly in prokaryotes like Escherichia coli. It plays a significant role in the homologous recombination pathway, which is vital for maintaining genomic stability, facilitating DNA repair, and ensuring proper chromosome segregation during cell division. The RecF protein serves as an essential component of the RecFOR complex, which is responsible for the loading of the RecA recombinase onto single-stranded DNA (ssDNA) regions that arise during replication stress or DNA damage. Research on the RecF protein has gained prominence due to its implication in understanding cellular responses to DNA damage, its interactions with other repair proteins, and its potential influence on antibiotic resistance mechanisms. Moreover, the study of RecF and its functional partners has broad implications for biotechnological applications, including the development of strategies for targeted genome editing and improvements in microbial strain engineering. By elucidating the molecular mechanisms governing DNA repair through RecF, researchers aim to uncover new avenues for therapeutic interventions against diseases linked to DNA repair deficiencies, such as cancer and genetic disorders. Overall, the RecF protein remains a focal point of molecular biology research, contributing to our understanding of DNA repair pathways and their significance in cellular integrity and evolution.
