Analytical Data
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基因名
DDOST
- Application
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别名
DDOST；KIAA0115；OST48；Dolichyl-diphosphooligosaccharide--Protein glycosyltransferase 48 kDa subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P39656
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表达区间
1-456aa
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氨基酸序列
MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVL LDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIF SPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFD EEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGV GMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQAR NNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVF KEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDG DDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYT HLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSIVFLHMK EKEKSD
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分子量
77 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDOST, or Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase, is an essential enzyme that plays a pivotal role in the N-glycosylation process, which is critical for protein folding and function in eukaryotic cells. Emerging as a significant focus in biochemical research, DDOST catalyzes the transfer of pre-assembled oligosaccharides onto nascent polypeptides within the endoplasmic reticulum, facilitating proper protein maturation and stability. Disturbances in DDOST function have been associated with various diseases, including genetic disorders and cancers, underscoring its importance in cellular homeostasis. The study of DDOST’s role in glycosylation is crucial for understanding how glycomic changes affect physiological and pathological processes. Furthermore, the potential therapeutic applications of manipulating DDOST activity or its pathways to treat related diseases highlight the importance of ongoing research in this area. By creating recombinant forms of DDOST, scientists aim to elucidate its structural and functional properties, leading to innovations in targeted therapies and biotechnological applications. The investigation of DDOST also promises to provide insights into glycosylation patterns that could serve as biomarkers for disease diagnosis and treatment stratification. As such, the exploration of DDOST as a protein model forms a critical dimension in the fields of glycobiology, biochemistry, and pharmaceutical science, paving the way for advances in understanding complex biological systems and developing novel therapeutic strategies.
