Analytical Data
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基因名
CCT5
- Application
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别名
CCT 5; CCT epsilon ; CCT-epsilon; CCT5; CCTE; CCTepsilon; Chaperonin containing T-complex polypeptide 1; subunit 5; Chaperonin containing TCP 1 epsilon; Chaperonin containing TCP 1 subunit 5; Chaperonin containing TCP 1 subunit 5 epsilon
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48643
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表达区间
1-541aa
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氨基酸序列
MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE
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分子量
85.25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CCT5, a member of the chaperonin-containing TCP1 (CCT) complex, plays a crucial role in protein folding and assembly within the cytosol of eukaryotic cells. This complex assists in the proper folding of various structural proteins, including actins and tubulins, which are essential for maintaining cellular integrity and function. Research on CCT5 is driven by its potential implications in diseases associated with protein misfolding and aggregation, such as neurodegenerative disorders and cancer. Understanding the mechanistic intricacies of CCT5 can provide insights into its function in cellular homeostasis and stress responses. Moreover, the recombinant expression of CCT5 can facilitate the study of its structure-function relationship and its interactions with substrate proteins, ultimately contributing to the development of therapeutic strategies aimed at modulating protein folding pathways. Additionally, given the evolutionarily conserved nature of CCT proteins, findings from CCT5 research may extend to broader biological contexts, enhancing our comprehension of proteomic stability and cellular resilience across different species. Therefore, investigating CCT5 as a recombinant protein not only emphasizes its biochemical significance but also underscores the potential for innovating treatment modalities for protein misfolding-related diseases.
