Analytical Data
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基因名
ECE2
- Application
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别名
ECE2；KIAA0604；Endothelin-converting enzyme 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DPD8
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表达区间
200-883aa
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氨基酸序列
GVQYHRDPS HSTCLTEACI RVAGKILESL DRGVSPCEDF YQFSCGGWIR RNPLPDGRSR WNTFNSLWDQ NQAILKHLLE NTTFNSSSEA EQKTQRFYLS CLQVERIEEL GAQPLRDLIE KIGGWNITGP WDQDNFMEVL KAVAGTYRAT PFFTVYISAD SKSSNSNVIQ VDQSGLFLPS RDYYLNRTAN EKVLTAYLDY MEELGMLLGG RPTSTREQMQ QVLELEIQLA NITVPQDQRR DEEKIYHKMS ISELQALAPS MDWLEFLSFL LSPLELSDSE PVVVYGMDYL QQVSELINRT EPSILNNYLI WNLVQKTTSS LDRRFESAQE KLLETLYGTK KSCVPRWQTC ISNTDDALGF ALGSLFVKAT FDRQSKEIAE GMISEIRTAF EEALGQLVWM DEKTRQAAKE KADAIYDMIG FPDFILEPKE LDDVYDGYEI SEDSFFQNML NLYNFSAKVM ADQLRKPPSR DQWSMTPQTV NAYYLPTKNE IVFPAGILQA PFYARNHPKA LNFGGIGVVM GHELTHAFDD QGREYDKEGN LRPWWQNESL AAFRNHTACM EEQYNQYQVN GERLNGRQTL GENIADNGGL KAAYNAYKAW LRKHGEEQQL PAVGLTNHQL FFVGFAQVWC SVRTPESSHE GLVTDPHSPA RFRVLGTLSN SRDFLRHFGC PVGSPMNPGQ LCEVW
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分子量
80 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ECE2 (Endothelin-converting enzyme 2) is a membrane-bound metalloprotease that plays a pivotal role in the processing of endothelins, which are key regulators of various physiological processes, including blood pressure, vascular tone, and cell proliferation. The study of ECE2 is particularly important due to its implications in cardiovascular diseases and other pathological conditions. This enzyme is responsible for the cleavage of big endothelin-1 into its active form, endothelin-1, which has potent vasoconstrictive properties. However, ECE2 is not just limited to endothelin processing; it also participates in the metabolism of other peptide substrates, adding to its biological significance. Research into the ECE2 protein has deepened our understanding of its structural and functional characteristics, including the identification of its active sites and substrate specificity. Furthermore, advances in recombinant protein technology have facilitated the production of ECE2 for in vitro studies, enabling scientists to investigate its enzymatic activity, regulatory mechanisms, and potential as a therapeutic target. Given the enzyme's association with conditions such as hypertension, heart failure, and cancer, ongoing research aims to explore ECE2 inhibitors as potential treatment strategies. Therefore, unraveling the complexities of ECE2 not only enhances our knowledge of cardiovascular biology but also holds promise for the development of novel therapeutic approaches.
