Analytical Data
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基因名
CDKL5
- Application
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别名
Cdkl5; CDKL5_HUMAN; Cyclin dependent kinase 5 transcript; Cyclin-dependent kinase-like 5; EIEE2; ISSX; Serine/threonine kinase 9; Serine/threonine-Protein kinase 9; Stk9
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O76039
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表达区间
722-831aa
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氨基酸序列
RPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPAFDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQT
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分子量
37.73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDKL5 (Cyclin-Dependent Kinase-Like 5) is a serine/threonine kinase that plays a crucial role in neuronal development and synaptic function. Mutations in the CDKL5 gene are linked to CDKL5 deficiency disorder (CDD), a severe form of neurodevelopmental disorder characterized by intractable epilepsy, intellectual disability, and developmental delays, primarily affecting females. Research on CDKL5 recombinant protein has gained significant attention due to its potential in elucidating the molecular mechanisms underlying CDD and exploring therapeutic avenues. The recombinant protein can be used to study the kinase's role in cellular signaling pathways, interactions with other proteins, and effects on neuronal maturation. Additionally, characterizing CDKL5's enzymatic activity and its substrates can provide insights into how its dysfunction leads to the phenotypic manifestations of CDD. The development of animal models expressing CDKL5 mutations further facilitates the assessment of potential interventions, including gene therapy and pharmacological strategies aimed at rescuing the loss of CDKL5 function. Overall, the study of CDKL5 recombinant protein is pivotal in advancing our understanding of CDD and developing targeted treatments for affected individuals.
