Analytical Data
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Gene name
SF3B5
- Application
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Alternative Names
SF3B5; SF3B10; Splicing factor 3B subunit 5; SF3b5; Pre-mRNA-splicing factor SF3b 10 kDa subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BWJ5
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Expression Region
2-86 aa
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AA Sequence
TDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN
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Molecular Weight
10.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The SF3B5 protein, a component of the spliceosome, plays a crucial role in the pre-mRNA splicing process, which is essential for the proper expression of genes in eukaryotic cells. Alterations in splicing mechanisms can lead to various diseases, including cancers and genetic disorders. As a member of the SF3B protein complex, SF3B5 is involved in the recognition of splice sites, facilitating the removal of introns and the joining of exons. Research has shown that mutations in the SF3B5 gene can affect splicing fidelity, leading to aberrant protein isoforms that contribute to disease pathology. Given its importance, the recombinant SF3B5 protein serves as a valuable tool for studying splicing regulation and understanding disease mechanisms. Advances in protein expression technologies have enabled the production of functional recombinant SF3B5, allowing researchers to investigate its biophysical properties, interactions with other splicing factors, and its role in various cellular contexts. Furthermore, this research may pave the way for therapeutic strategies aimed at correcting splicing defects, highlighting the significance of SF3B5 in both basic and applied biomedical research.
