Analytical Data
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基因名
CPSF5
- Application
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别名
CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43809
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表达区间
1-227aa
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氨基酸序列
MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
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分子量
50.71 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CPSF5, also known as cleavage and polyadenylation specificity factor subunit 5, plays a crucial role in the post-transcriptional regulation of gene expression by participating in the polyadenylation of mRNA precursors. This protein is a component of the CPSF complex, which is essential for the cleavage of pre-mRNA and the addition of a poly(A) tail, a modification that stabilizes mRNA and facilitates its export from the nucleus to the cytoplasm. Alterations in CPSF5 function can lead to various cellular dysfunctions and have been implicated in multiple diseases, including cancers and genetic disorders. The study of CPSF5 as a recombinant protein enables researchers to dissect its specific interactions and regulatory mechanisms in greater detail, facilitating the understanding of its role in mRNA processing and stability. Additionally, the ability to produce and purify CPSF5 in vitro allows for the development of potential therapeutic strategies aimed at modulating gene expression through the manipulation of mRNA polyadenylation dynamics. Thus, ongoing research into CPSF5 is critical for elucidating the complexities of gene regulation and may offer insights into novel approaches for treating diseases associated with dysregulated gene expression.
