Analytical Data
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基因名
OXCT1
- Application
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别名
OXCT1；OXCT；SCOT；Succinyl-CoA:3-ketoacid coenzyme A transferase 1. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P55809
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表达区间
40-520aa
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氨基酸序列
TKFYTDPVEAVKDIPDGATVLVGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN
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分子量
56.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OXCT1, or 3-oxoacid CoA transferase 1, is an important enzyme involved in the metabolism of ketone bodies, particularly in the utilization of acetoacetate and its conversion into acetyl-CoA, which is crucial for energy production in various tissues, including the brain and heart. Recent studies have highlighted the significance of OXCT1 in metabolic health, as it plays a pivotal role in ketone body utilization during periods of fasting or low carbohydrate intake. Alterations in OXCT1 expression or activity have been linked to metabolic disorders such as obesity, diabetes, and neurological diseases, underscoring its potential as a therapeutic target. Research on recombinant OXCT1 protein aims to elucidate its biochemical properties, regulatory mechanisms, and interactions with other metabolic pathways. By producing this protein in a controlled laboratory setting, scientists can investigate its enzymatic activity, structural characteristics, and influence on cellular metabolism. This research not only enhances our understanding of OXCT1's role in energy homeostasis but also paves the way for developing strategies to modulate its function for therapeutic purposes, potentially offering new avenues for treating metabolic diseases associated with dysregulated ketone metabolism.
