Analytical Data
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Gene name
Spink2
- Application
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Alternative Names
Spink2；Serine protease inhibitor Kazal-type 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20155
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Expression Region
1-84aa
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AA Sequence
MALSVLRLAL LLLAVTFAAS LIPQFGLFSK YRTPNCSQYR LPGCPRHFNP VCGSDMSTYA NECTLCMKIR EGGHNIKIIR NGPC
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Molecular Weight
34 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Spink2, or Serine Protease Inhibitor Kazal-type 2, is a member of the Kazal-type serine protease inhibitor family, which plays a crucial role in the regulation of serine proteases that are vital in various physiological processes. This protein has garnered significant attention due to its involvement in several pathological conditions, including pancreatitis, inflammation, and certain cancers. Researchers aim to understand the functional dynamics of Spink2 and its mechanisms of action to elucidate its contribution to disease pathogenesis. Recombinant Spink2 protein serves as a valuable tool for investigating its biochemical properties, interaction with target proteases, and potential therapeutic applications. The production and characterization of recombinant Spink2 enable detailed studies into its inhibitory effects on serine proteases and its role in modulating inflammatory responses. Additionally, understanding the structural features of Spink2 through advanced techniques such as X-ray crystallography and NMR spectroscopy can provide insights into its stability and binding affinities. Overall, the research on Spink2 recombinant protein is crucial for developing novel strategies to treat diseases related to protease dysregulation and for advancing our knowledge of protease inhibition mechanisms in cellular contexts.
