Analytical Data
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基因名
WRKY2
- Application
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别名
WRKY2；Probable WRKY transcription factor 2
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9FG77
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表达区间
175-405aa
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氨基酸序列
FHPVSRSSSSFFQGTTEMMSVDYGNYNNRSSSHQSAEEVKPGSENIESSNLYGIETDNQNGQNKTSDVTTNTSLETVDHQEEEEEQRRGDSMAGGAPAEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKVERSREGHITEIIYKGAHNHLKPPPNRRSGMQVDGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNENNVEEGSTRFEYGNQSGSIQAQTGGQYESGD
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分子量
27.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The WRKY2 protein is a member of the WRKY family, which plays a crucial role in plant defense mechanisms, particularly in response to various environmental stresses, including pathogens and abiotic factors. This transcription factor is characterized by the presence of one or two conserved WRKY domains and a zinc finger motif, which facilitate its binding to specific DNA sequences in the promoters of target genes. Research has increasingly focused on WRKY2 due to its involvement in regulating genes associated with defense responses and its potential role in enhancing plant resilience. Various studies indicate that WRKY2 acts as a positive or negative regulator in stress responses, depending on the context and external stimuli. Understanding the functional dynamics of WRKY2 is essential for unraveling the complex signaling pathways that govern plant responses to stressors. Furthermore, there is a growing interest in the application of WRKY2 in agricultural biotechnology, aiming to develop crops with improved stress tolerance through genetic engineering approaches. Therefore, recombinant protein studies of WRKY2 provide valuable insights into its structure-function relationships and regulatory mechanisms, paving the way for novel strategies to enhance crop resilience and productivity in the face of climate change and increasing environmental challenges.
