Cat: PA1000-8318

Recombinant Human PBRM1 Protein,His

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Analytical Data

  • Gene name

    PBRM1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    PBRM1;BAF180;PB1;Protein polybromo-1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q86U86

  • Expression Region

    53-345aa

  • AA Sequence

    LYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAI

  • Molecular Weight

    34 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

PBRM1 (Polybromo-1) is a critical component of the PBAF (Polybromo-associated Brg1-A-containing factor) chromatin remodeling complex, which plays a significant role in gene regulation and chromatin dynamics. Research on PBRM1 has gained prominence due to its involvement in various cellular processes, including cell cycle regulation, DNA repair, and transcriptional activation. Mutations and aberrations in PBRM1 have been linked to several cancers, particularly renal cell carcinoma, suggesting its potential as a tumor suppressor. The study of PBRM1-recombinant proteins enables deeper insights into its structural and functional characteristics, providing a platform for understanding how it interacts with other components of the chromatin remodeling complex. Additionally, investigating PBRM1's role in epigenetic regulation may reveal novel therapeutic targets and strategies for cancer treatment. Given the complexity of its interactions within the chromatin landscape, the generation of purified PBRM1 recombinant proteins is essential for biochemical assays, structural biology studies, and functional analyses, further highlighting its significance in cancer biology and epigenetics.

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