Analytical Data
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Gene name
espK
- Application
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Species
Mycobacterium tuberculosis
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P9WJC1
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Expression Region
21-114aa
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Molecular Weight
17.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of espK recombinant protein is situated within the broader context of bacterial pathogenesis and host immunity. EspK, a protein secreted by certain pathogenic strains of Escherichia coli, plays a crucial role in the manipulation of host cellular processes. This protein is part of the Type III secretion system (T3SS), a sophisticated mechanism that allows these bacteria to inject virulence factors directly into host cells, thereby facilitating infection and evasion of the immune response. Understanding espK's structure and function is vital for elucidating the molecular mechanisms underlying E. coli pathogenicity. Research has indicated that espK may influence key host signaling pathways, potentially leading to dysregulation of immune responses. As such, investigating espK offers insights not just into bacterial infection strategies but also into potential therapeutic avenues, including the development of vaccines or targeted treatments. Furthermore, recombinant expression of espK in laboratory settings allows researchers to analyze its interaction with host proteins and assess its effects on cellular processes, making it an important focus in the fields of microbiology, immunology, and infectious disease research. By advancing our understanding of espK and its role in pathogenesis, we may uncover new strategies to combat bacterial infections, enhancing public health outcomes.
