Analytical Data
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基因名
RhoD
- Application
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别名
RHOD;ARHD;Rho-related GTP-binding Protein RhoD
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00212
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表达区间
18-207aa
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氨基酸序列
MMGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C
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分子量
24 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RhoD is a member of the Rho family of GTPases, which are known to play a crucial role in regulating various cellular processes, including cytoskeletal dynamics, cell adhesion, and migration. Research on RhoD has gained momentum due to its involvement in the actin cytoskeleton reorganization and its potential implications in several physiological and pathological conditions, such as cancer metastasis and neurodegenerative diseases. Unlike other Rho GTPases, RhoD exhibits distinct regulatory mechanisms and signaling pathways, which have made it a focus of interest in cellular biology and therapeutic development. The study of RhoD recombinant proteins is essential for elucidating its molecular functions, interactions with other cellular components, and its regulatory mechanisms. Furthermore, understanding RhoD's precise role in cellular processes can provide insights into novel therapeutic targets and strategies for diseases associated with dysregulated actin dynamics. Recent advances in molecular biology techniques have enabled the production of recombinant RhoD proteins, facilitating in vitro studies and biochemical assays that aim to decipher its function and regulation. Overall, the ongoing research on RhoD recombinant proteins aims to unveil the intricacies of its biological roles and the potential clinical applications that arise from targeting its signaling pathways.
