Analytical Data
-
Gene name
ALDH7A1
- Application
-
Alternative Names
ALDH7A1；ATQ1；Alpha-aminoadipic semialdehyde dehydrogenase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P49419
-
Expression Region
27-539aa
-
AA Sequence
AFMS TLLINQPQYA WLKELGLREE NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWK IWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY VDICDYAVGL SRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV YGWNNAIAMI CGNVCLWKGA PTTSLISVAV TKIIAKVLED NKLPGAICSL TCGGADIGTA MAKDERVNLL SFTGSTQVGK QVGLMVQERF GRSLLELGGN NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHE SIHDEVVNRL KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSKDL PLAQGIKFQ
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALDH7A1, or Aldehyde Dehydrogenase 7 Family Member A1, is a crucial enzyme in the detoxification of endogenous aldehydes and plays a significant role in the metabolism of neurotransmitters and amino acids. Mutations in the ALDH7A1 gene are linked to pyridoxine-dependent epilepsy (PDE), a rare but severe neurological disorder that presents in infancy with seizures unresponsive to conventional treatments. The characterization and production of recombinant ALDH7A1 protein are essential for understanding its enzymatic functions, elucidating the mechanisms by which its deficiency leads to pathology, and developing potential therapeutic strategies. By generating this recombinant protein, researchers can explore its substrate specificity, inhibition characteristics, and the impact of various mutations associated with PDE. Furthermore, studies utilizing the recombinant enzyme may help in the design of small molecule activators or gene therapy approaches to restore functional activity in affected individuals. The research on ALDH7A1 not only enhances our fundamental understanding of enzyme biology but also has significant implications for the development of targeted treatments for individuals suffering from pyridoxine-dependent epilepsy and related disorders.
