Analytical Data
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基因名
YARS1
- Application
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别名
YARS1；YARS；Tyrosine--tRNA ligase. cytoplasmic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54577
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表达区间
2-528aa
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氨基酸序列
GDAPSPEEKLHLITRNLQEVLGEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPMAKGPAKNSEPEEVIPSRLDIRVGKIITVEKHPDADSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSISCKSLKGGNIS
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分子量
86.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
YARS1, or tyrosyl-tRNA synthetase 1, is an essential enzyme involved in the protein synthesis mechanism by catalyzing the attachment of the amino acid tyrosine to its corresponding transfer RNA (tRNA). This process is crucial for generating functional proteins within cells. Research on YARS1 has garnered significant attention due to its implications in various biological processes and diseases, including cancer and autoimmune disorders. Abnormalities in YARS1 expression or activity can lead to dysregulation of protein synthesis, contributing to cellular malfunction and disease progression. Additionally, YARS1 has been implicated in the maturation of regulatory RNA molecules and plays a role in innate immunity responses. Understanding the structure and function of YARS1, as well as the mechanisms governing its activity, provides valuable insights for potential therapeutic interventions. Researchers are increasingly focused on investigating the reactivity, specificity, and interaction of YARS1 with other cellular components to uncover its multifaceted roles in health and disease. The development of YARS1 recombinant proteins for experimental purposes has opened avenues for studying its biochemical properties and interactions, and has potential implications for the design of targeted treatments that could modulate its function. Overall, YARS1 represents a significant focal point in the study of aminoacyl-tRNA synthetases and their regulatory roles in cellular physiology and pathology.
