Analytical Data
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基因名
ZP3
- Application
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别名
ZP3；ZP3A；ZP3B；ZPC；Zona pellucida sperm-binding Protein 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P21754
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表达区间
23-387aa
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氨基酸序列
QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLT LGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRP VGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTF SLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPT PDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFAN DSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCC NKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRG DHEVEQWALPSDTSV
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZP3, or zona pellucida glycoprotein 3, is a crucial protein involved in the fertilization process in mammals. It is a component of the zona pellucida, the glycoprotein membrane surrounding oocytes, and plays a significant role in sperm-egg recognition and binding. Research on recombinant ZP3 proteins has gained momentum due to their potential applications in reproductive biotechnology, such as in vitro fertilization (IVF) and contraceptive development. The interest in ZP3 is further fueled by its immunological properties, as it can elicit specific antibody responses that may be leveraged for immunocontraceptive strategies. Additionally, studying the structure and function of ZP3 can provide insights into the mechanisms of fertilization and potential reproductive disorders. As a recombinant protein, ZP3 can be produced in various expression systems, enabling the detailed investigation of its functional characteristics and interactions with sperm. Overall, the study of ZP3 recombinant proteins is significant for advancements in reproductive biology, fertility treatments, and the development of novel contraceptive methods.
