Analytical Data
-
基因名
Pka-C1
- Application
-
别名
Pka-C1；PKACA；cAMP-dependent Protein kinase catalytic subunit alpha
-
种属
E.coli
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P12370
-
表达区间
2-353aa
-
氨基酸序列
GNNATTSNKKVDAAETVKEFLEQAKEEFEDKWRRNPTNTAALDDFERIKTLGTGSFGRVMIVQHKPTKDYYAMKILDKQKVVKLKQVEHTLNEKRILQAIQFPFLVSLRYHFKDNSNLYMVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYLHYLDLIYRDLKPENLLIDSQGYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKNQKWFASTDWIAIFQKKIEAPFIPRCKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF
-
分子量
56.7 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Pka-C1, or Protein kinase A catalytic subunit 1, is a key enzyme involved in the cAMP-dependent signaling pathway, which plays a crucial role in various cellular processes such as metabolism, gene expression, and cell proliferation. The research background of Pka-C1 focuses on understanding its structure-function relationships, regulatory mechanisms, and the implications of its dysregulation in diseases. Abnormal Pka-C1 activity has been linked to several pathological conditions, including cancer, heart diseases, and neurodegenerative disorders, making it a significant target for therapeutic interventions. Recent advances in recombinant protein technology have enabled the efficient production and purification of Pka-C1, facilitating detailed biochemical studies and structure determination through techniques such as X-ray crystallography and NMR spectroscopy. Researchers are particularly interested in the development of specific inhibitors that could modulate Pka-C1 activity, advancing our understanding of its biological roles and potential as a drug target. As a result, Pka-C1 continues to be a subject of intense investigation in the fields of biochemistry, pharmacology, and molecular biology, with the aim of elucidating its function in health and disease.
