Analytical Data
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基因名
TF
- Application
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别名
TF;Tissue factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P13726
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表达区间
1-295aa
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氨基酸序列
METPAWPRVPRPETAVARTLLLGWVFAQVAGASGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFREIFYIIGAVVFVVIILVIILAISLHKCRKAGVGQSWKENSPLNVS
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on transcription factor (TF) recombinant proteins has gained significant attention due to the pivotal role that transcription factors play in regulating gene expression and influencing various biological processes. Transcription factors are proteins that bind to specific DNA sequences, thereby controlling the transcription of genetic information from DNA to messenger RNA. Their activity is critical in processes such as cell differentiation, development, and response to environmental stimuli. However, studying the complex mechanisms of TFs often poses challenges, as they typically function in concert within intricate cellular networks. The advent of recombinant DNA technology has enabled the production of TFs in a controlled environment, allowing researchers to generate large quantities of pure proteins. This has facilitated the exploration of their structure-function relationships, interactions with other proteins, and regulatory pathways. Additionally, recombinant TFs are valuable tools in synthetic biology and therapeutic applications, such as gene therapy and the development of targeted cancer treatments. By engineering TFs with altered specificity or enhanced activity, scientists aim to manipulate cellular processes for desired outcomes. Overall, the ongoing research in TF recombinant proteins holds promise for advancing our understanding of gene regulation and for developing innovative strategies to combat various diseases.












