Analytical Data
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基因名
virE2
- Application
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别名
virE2；Single-strand DNA-binding Protein
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A3W9
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表达区间
316-533aa
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氨基酸序列
NRAHNRQFPTATVNMGQQPDGQGGLTRDRHVSVEFLMQSAPNSPWAQALKKGELWDRVQLLARDGNRYLSPHRLEYSDPEHFTELMNRVGLPASMGRQSHAASIKFEKFDAQAAVIVINGPELRDIHDLSPENLQNVSTKDVIVADRNENGQRTGTYTSVAEYERLQLRLPADAAGVLGEAADKYSRDFVRPEPASRPISDSRRIYESRPRSQSVNSF
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分子量
31.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VirE2 protein, encoded by the Agrobacterium tumefaciens pTi virulence plasmid, plays a crucial role in the transformation of plant cells by acting as a key component in the delivery of T-DNA into the host genome. Understanding the structure and function of VirE2 is essential for exploiting its properties in biotechnology and genetic engineering. Research has shown that VirE2 forms a complex with T-DNA during its transport, facilitating nuclear import and integration into the plant's DNA. This interaction is vital for the Agrobacterium-mediated genetic transformation process, which has significant applications in generating transgenic plants and improving agricultural traits. Investigating the biochemical properties and interactions of VirE2 can enhance our understanding of its mechanism of action and enable the development of novel tools for plant transformation. Furthermore, the potential use of VirE2 as a vector for delivering genetic material into eukaryotic cells expands its significance beyond plant biology into broader fields such as medicine and biotechnology. Recent studies focus on the optimization of VirE2 for increased efficiency and specificity in delivering therapeutic genes, highlighting its importance in the advancement of gene therapy methods. Overall, the exploration of VirE2 as a recombinant protein not only sheds light on fundamental plant-pathogen interactions but also opens avenues for innovative biotechnological applications.
