Analytical Data
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基因名
SEMA4D
- Application
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别名
SEMA4D；C9orf164；CD100；SEMAJ；Semaphorin-4D
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q92854
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表达区间
22-734aa
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氨基酸序列
MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVF AVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSAT SLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDG ELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSP DSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKW TSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVG LSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACI DSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYT QIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFE PVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCA WSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGT AELKCSQKSNLARVFWKFQNDVLKAESPKYGLMGRKNLLIFNLSEGDSGV YQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATK VLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLH SEKTMYLKSSDNR
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分子量
105 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SEMA4D, or Semaphorin 4D, is a member of the semaphorin family, which plays a crucial role in various biological processes, including neural development, immune response, and tumorigenesis. Initially identified for its role in axon guidance during nervous system development, SEMA4D has garnered significant attention due to its involvement in cancer biology. It is expressed in various tissues and is particularly noted for its effects on cell adhesion, migration, and the immune system's regulation. In the tumor microenvironment, SEMA4D can modulate immune cell trafficking and function, contributing to tumor growth and metastasis. Furthermore, studies have suggested that SEMA4D interacts with its receptors, such as Plexin-B1 and Neuropilin-1, to influence signaling pathways that affect angiogenesis and immune evasion in cancers. Understanding the structure-function relationship of SEMA4D and its signaling mechanisms has emerged as a vital area of research, with potential implications for therapeutic interventions in cancer and other diseases. Investigating the recombinant forms of SEMA4D can provide insights into its biological activities and help develop targeted therapies that could inhibit its pro-tumorigenic effects, paving the way for innovative cancer treatment strategies.
