Analytical Data
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Gene name
FBXL3
- Application
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Alternative Names
FBXL3；FBL3A；FBXL3A；F-box/LRR-repeat Protein 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKT7
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Expression Region
1-428aa
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AA Sequence
MKRGGRDSDR NSSEEGTAEK SKKLRTTNEH SQTCDWGNLL QDIILQVFKY LPLLDRAHAS QVCRNWNQVF HMPDLWRCFE FELNQPATSY LKATHPELIK QIIKRHSNHL QYVSFKVDSS KESAEAACDI LSQLVNCSLK TLGLISTARP SFMDLPKSHF ISALTVVFVN SKSLSSLKID DTPVDDPSLK VLVANNSDTL KLLKMSSCPH VSPAGILCVA DQCHGLRELA LNYHLLSDEL LLALSSEKHV RLEHLRIDVV SENPGQTHFH TIQKSSWDAF IRHSPKVNLV MYFFLYEEEF DPFFRYEIPA THLYFGRSVS KDVLGRVGMT CPRLVELVVC ANGLRPLDEE LIRIAERCKN LSAIGLGECE VSCSAFVEFV KMCGGRLSQL SIMEEVLIPD QKYSLEQIHW EVSKHLGRVW FPDMMPTW
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FBXL3 (F-box and leucine-rich repeat protein 3) is a critical component of the ubiquitin-proteasome system, playing a vital role in the regulation of protein degradation and cellular homeostasis. Recent studies have highlighted its involvement in circadian rhythm regulation, whereby FBXL3 acts as a substrate recognition component for the E3 ubiquitin ligase complex, targeting specific proteins for degradation. Aberrations in FBXL3 activity have been linked to various pathologies, including sleep disorders and metabolic syndromes. Additionally, its role in modulating the stability of essential clock proteins like CRY1 and CRY2 underscores its significance in maintaining circadian clock functions. With the growing interest in the circadian biology field, harnessing recombinant FBXL3 proteins has become crucial for elucidating their biochemical pathways, understanding their interactions with other clock proteins, and exploring their therapeutic potential in circadian-related diseases. Thus, research on FBXL3 recombinant proteins offers vital insights into the mechanisms underlying circadian regulation and its broader implications for health and disease.
