Analytical Data
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Gene name
CHMP4B
- Application
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Alternative Names
CHMP4B；C20orf178；SHAX1；Charged multivesicular body Protein 4b
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H444
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Expression Region
1-224aa
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AA Sequence
SVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM
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Molecular Weight
51.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHMP4B, a member of the CHMP (charged multivesicular body protein) family, plays a crucial role in the endosomal sorting of proteins and the biogenesis of multivesicular bodies (MVBs). This protein is essential for the formation of intraluminal vesicles within MVBs, facilitating the degradation of membrane proteins and the sorting of proteins destined for exosomes. Research indicates that CHMP4B is implicated in various cellular processes, including the regulation of the intracellular pathways leading to immune responses and viral infections. Mutations or dysregulation of CHMP4B have been linked to several diseases, including cancer and neurodegenerative disorders, suggesting its potential role as a therapeutic target. Understanding the structure and function of CHMP4B is pivotal for elucidating its mechanisms in cellular compartmentalization and protein trafficking, which are vital for maintaining cellular homeostasis. Recent advances in recombinant protein technology have enabled the production and purification of CHMP4B, facilitating detailed studies of its interactions with other cellular proteins and its role in the endosomal-lysosomal pathway. By investigating CHMP4B through recombinant protein studies, researchers aim to provide insights into its biological functions and therapeutic potential, offering avenues for developing novel strategies in disease intervention.
