Analytical Data
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Gene name
Glypican-3/GPC3
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简介
The Glypican-3 (GPC3) protein is a cell surface proteoglycan that plays complex regulatory roles in key signaling pathways critical to developmental processes. Through its GPI anchoring, GPC3 negatively regulates the Hedgehog signaling pathway by competing with the Hedgehog receptor PTC1 for binding to Hedgehog protein, leading to complex internalization and subsequent lysosomal degradation. PE-labeled Glypican-3/GPC3 Protein, Human (HEK293, His) is the recombinant human-derived PE-labeled Glypican-3/GPC3 protein, expressed by HEK293 , with His labeled tag.
- Application
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Alternative Names
GPC3; OCI5; Glypican-3; GTR2-2; MXR7; DGSX; SDYS ; SGB; SGBS; SGBS1
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Species
Human
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Source
HEK293
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Tag
His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51654-1
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Expression Region
Q25-H559
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Protein Length
Partial
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Molecular Weight
64.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glypican-3 (GPC3) is a heparan sulfate proteoglycan that plays a crucial role in cell growth and development, particularly in the context of various cancers. It is predominantly expressed in fetal tissues and is typically downregulated in normal adult tissues, making it a significant marker for certain tumors, especially hepatocellular carcinoma (HCC) and Wilms' tumor. The aberrant expression of GPC3 in tumors is associated with poor clinical outcomes, highlighting its potential as a therapeutic target and a diagnostic biomarker. Research into GPC3 has intensified due to its unique characteristics; it modulates signaling pathways such as Wnt, Hedgehog, and insulin-like growth factor, which are crucial for cell proliferation and differentiation. As a result, recombinant GPC3 protein has been developed for various applications, including targeted therapies and vaccine development against GPC3-expressing tumors. The study of GPC3 also opens avenues for understanding its role in tumor microenvironments and interactions with other cellular receptors, aiming to harness its properties for developing novel cancer treatments. Given its pivotal role in oncogenesis and tumor progression, GPC3 remains a focal point of ongoing research efforts in the fields of oncology and regenerative medicine.
