Analytical Data
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Gene name
BMP-15
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简介
Bone morphogenetic protein 15 (BMP-15; GDF9B), also known as growth differentiation factor 9B (GDF9B), is a polymorphic ligand protein of the TGFβ family and is only expressed in follicular cells. BMP15 is closely related to GDF9 and synergistically regulates the genetic development of follicles. BMP15 is involved in p38 MAPK and HIF-1α/SCF signaling pathway, respectively, and can up-regulate the expression of anti-Mullerian hormone (AMH) and polycystic ovarian syndrome (PCOS) related stem cell factor (SCF) in granulosa cells.
- Application
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Biological Activity
Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation. Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.
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Alternative Names
Growth/differentiation factor 9B
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Species
Human
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Source
E. coli
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Tag
C-Myc;N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95972
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Expression Region
Q268-R392
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Protein Length
Full Length of Mature Protein
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Molecular Weight
23 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BMP-15 (Bone Morphogenetic Protein 15) is a member of the transforming growth factor-beta (TGF-β) superfamily, primarily expressed in the ovaries and known for its crucial role in female reproduction, particularly in oocyte development and folliculogenesis. Research has shown that BMP-15 plays a significant role in regulating ovarian function and fertility, influencing granulosa cell proliferation and differentiation. Mutations in the BMP-15 gene have been associated with various reproductive disorders, making it a focal point of study for understanding infertility and genetic factors affecting reproductive health. Additionally, BMP-15 has garnered attention in the field of biotechnology and reproductive medicine, as its recombinant protein can potentially be used for clinical applications in ovarian stimulation protocols, enhancing fertility treatments. Advances in recombinant DNA technology have allowed for the production of BMP-15 in vitro, facilitating further exploration of its biological functions and therapeutic potential. Ongoing research aims to elucidate the signaling pathways involved in BMP-15 action, its interactions with other reproductive hormones, and its potential implications in assisted reproductive technologies (ART). By comprehensively understanding BMP-15’s mechanisms, scientists hope to develop novel strategies to improve fertility outcomes and treat reproductive disorders in women.
