Analytical Data
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Gene name
Nicastrin
- Application
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Alternative Names
APH2; NCT
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8CGU6
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Expression Region
Val35~Gln534
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Molecular Weight
59kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Nicastrin is a crucial component of the gamma-secretase complex, which plays a significant role in the proteolytic processing of various type I membrane proteins, including the amyloid precursor protein (APP). The dysregulation of gamma-secretase activity is implicated in several neurodegenerative diseases, particularly Alzheimer's disease, where improper cleavage of APP leads to the accumulation of beta-amyloid peptides, a hallmark of the pathology. Research on recombinant Nicastrin protein focuses on elucidating its structure and function within the gamma-secretase complex, which involves intricate interactions with other subunits such as presenilins, Aph-1, and Pen-2. Understanding the mechanisms by which Nicastrin contributes to substrate recognition and complex assembly can provide insights into disease mechanisms and therapeutic target identification. Additionally, studies on recombinant Nicastrin can aid in the development of small molecule inhibitors that specifically modulate gamma-secretase activity, potentially offering innovative strategies for the treatment of Alzheimer's disease and other related disorders. This research is vital for advancing our knowledge of gamma-secretase function and its implications in neurodegeneration.
