Analytical Data
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Gene name
WTAP
- Application
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Alternative Names
hFL(2)D; Pre-mRNA-Splicing Regulator WTAP; Female-lethal(2)D homolog; WT1-associated protein
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15007
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Expression Region
Met1~Asp150
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Molecular Weight
21kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WTAP (Wilms' tumor 1-associating protein) is an essential component of the RNA splicing machinery and plays a crucial role in the regulation of RNA metabolism. It is known for its involvement in the processing of mRNA and its functions in the biogenesis of small nuclear ribonucleoproteins (snRNPs). Studies have increasingly shown that WTAP is implicated in various biological processes, including cell proliferation, differentiation, and response to stress. Abnormal expression or mutations in the WTAP gene are linked to several types of human cancers, highlighting its potential as a therapeutic target and biomarker. Given its multifaceted roles in tumorigenesis and the regulation of alternative splicing events, the research on WTAP recombinant protein has gained significant attention. Scientists aim to characterize the protein’s structure and functional domains to understand its interactions with other splicing factors and RNA molecules. Furthermore, investigating WTAP's post-translational modifications and their effects on its activity might reveal new regulatory mechanisms in RNA processing. The development of WTAP recombinant protein could facilitate high-throughput screening of small molecules or antibodies for cancer therapeutics and provide insights into RNA biology, which may lead to novel strategies for disease intervention. Thus, WTAP serves as a promising candidate for further study in the field of molecular biology and oncology.
