Analytical Data
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Gene name
Opn4
- Application
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Alternative Names
(Opsin-4)
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Species
Rat
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8R456
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Expression Region
1-474aa
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Molecular Weight
53.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Opn4, also known as the melanopsin gene, encodes a protein that plays a crucial role in the regulation of circadian rhythms and light sensitivity in mammals. Discovered in the context of photoreception, Opn4 is predominantly expressed in a unique subset of retinal ganglion cells known as intrinsically photosensitive retinal ganglion cells (ipRGCs), which are integral to non-image-forming visual functions. Research into Opn4 and its reconstituted protein focuses on understanding its biochemical properties and signaling mechanisms, as well as its potential implications in various physiological processes, including sleep-wake cycles, mood regulation, and seasonal behaviors. The study of Opn4 protein also sheds light on disorders related to circadian disruption, such as seasonal affective disorder and sleep disorders. By utilizing recombinant DNA technology to produce purified Opn4 protein, researchers aim to elucidate its structural dynamics, phototransduction pathways, and interactions with other cellular signaling molecules. This progress in Opn4 research not only advances our understanding of phototransduction but also opens avenues for therapeutic interventions aimed at modulating circadian rhythms and addressing related health issues. Thus, the exploration of Opn4 in the context of its recombinant protein provides valuable insights into the intricate relationship between light perception and physiological regulation.
